{"id":2203,"date":"2014-12-09T15:46:01","date_gmt":"2014-12-09T15:46:01","guid":{"rendered":"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/?page_id=1734"},"modified":"2021-01-26T14:04:41","modified_gmt":"2021-01-26T14:04:41","slug":"iain-perry","status":"publish","type":"page","link":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/the-team\/lab-alumni\/iain-perry\/","title":{"rendered":"Iain Perry"},"content":{"rendered":"<p>A graduate from Cardiff University, I Completed my PhD investigate altitude adaptations of earthworms identifying differential regulation and population based SNPs that had developed in just 500 years.<\/p>\n<p>http:\/\/orca.cf.ac.uk\/137601\/<\/p>\n<p>To date few have looked into how earthworms have adapted or acclimatised to the harsh and<br \/>\ndynamic environment of high altitude. In this work, I explore the terrestrial invertebrates,<br \/>\nearthworms that were found at high altitude on the volcanic island of Pico in the Azores<br \/>\n(Portugal) and at Les Deux Alpes in the French Alps. I initially identify species presence along an<br \/>\naltitudinal transect compare species diversity and lineage, before investigating gene regulatory<br \/>\ncontrol and genomic adaptation between high and low altitude populations to identify if high<br \/>\naltitude populations have acquired a genetic advantage to their low altitude cousin or if all<br \/>\nworms have it within them to survive if given time to acclimatise.<br \/>\nAltitudinal transects of two temperate-zone mountains were conducted, at Les Deux Alpes and<br \/>\nPico, to identify presence and abundance of species. The two most abundant species, Lumbricus<br \/>\nterrestris and Aporrectodea caliginosa, were investigated to identify diversity and species<br \/>\nlineage to determine which species better allowed for adaption and acclimatisation<br \/>\ninvestigations, that are not heavily influenced by deeply rooted species diversity. Having<br \/>\nidentified A. caliginosa in Pico as the most suitable candidate for investigating adaption and<br \/>\nacclimatisation with its low population diversity, an de novo genome assembly was developed<br \/>\nand annotated.<br \/>\nLive individuals of A. caliginosa from a high and a low altitude site on Pico were acclimatised to<br \/>\nstandard laboratory conditions for six months prior to experimental exposure to conditions<br \/>\nsimulating six climatic conditions for two weeks with temperature and oxygen as variables.<br \/>\nRNAseq was performed on the RNA taken from a body transect (including muscular, nerve and<br \/>\ngut tissues) of the exposed experimental worms, and differential gene expression was<br \/>\ncalculated and explored between the high and low altitude populations. Despite both<br \/>\npopulations normalising in identical soils for 6 months, high altitude individuals had a lower<br \/>\nresponse in gene expression than the Low altitude individuals and suggested an element of<br \/>\nepigenetic conditioning or adaption allowing a more plastic response to the changes in<br \/>\nconditions. In particular, HMGB1, a gene that is known for its roles in regulating environmental<br \/>\nresponses, had a comparatively lower expression in the high altitude population than the low<br \/>\naltitude population when exposed to simulated high altitude climatic stressors. SNP analysis<br \/>\nfrom transcriptomic sequences revealed the high altitude individuals had SNPs associated with<br \/>\ngenes that linked to directly to this gene indicating a level of adaption through SNPs and<br \/>\nacclimatisation through potential epigenetic priming within the high altitude population.<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3472\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png?resize=200%2C200\" alt=\"\" width=\"200\" height=\"200\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png?w=200 200w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png?resize=150%2C150 150w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png?resize=100%2C100 100w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2021\/01\/workphoto.png?resize=40%2C40 40w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/><\/a><\/p>\n<p>Previous work includes:<\/p>\n<p>Perry, I. A., Szeto, J., Isaacs, M.D., Watson, P.D., Rose, R., Gealy, E.C., Scofield, S., Hayes, A.J. (2017)\u00a0A novel technique for producing 3D printed scale models from microscope volume datasets for use in science education, outreach and engagement. in press. EMS Engineering Science Journal. in press<\/p>\n<p>Perry, I. A., Sexton, K. J., Prytherch, Z. C., Blum, J. L., Zelikoff, J. T., B\u00e9ruB\u00e9, K. A. (2017) An <i>in vitro<\/i> versus\u00a0<em>in vivo<\/em> toxicogenomic investigation of prenatal exposures to tobacco smoke. Applied In Vitro Toxicology. in press<\/p>\n<p>Benzonana, L. L., Perry, N. J. S., Watts, H. R., Yang, B., Perry, I. A., Coombs, C., Takata, M., Ma, D. (2013) Isoflurane, a commonly used volatile anesthetic, enhances renal cancer growth and malignant potential via the hypoxia-inducible factor cellular signaling pathway in vitro. Anesthesiology. 119(3) 593-605<\/p>\n<p>email: perryIA3@cardiff.ac.uk<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A graduate from Cardiff University, I Completed my PhD investigate altitude adaptations of earthworms identifying differential regulation and population based SNPs that had developed in just 500 years. http:\/\/orca.cf.ac.uk\/137601\/ To date few have looked into how earthworms have adapted or acclimatised to the harsh and dynamic environment of high altitude. In this work, I explore<\/p>\n","protected":false},"author":2954,"featured_media":0,"parent":1482,"menu_order":1,"comment_status":"open","ping_status":"open","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-2203","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P6t6rP-zx","meta_box":[],"_links":{"self":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/pages\/2203","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/users\/2954"}],"replies":[{"embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/comments?post=2203"}],"version-history":[{"count":10,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/pages\/2203\/revisions"}],"predecessor-version":[{"id":3485,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/pages\/2203\/revisions\/3485"}],"up":[{"embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/pages\/1482"}],"wp:attachment":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/media?parent=2203"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}