{"id":1808,"date":"2015-01-27T23:51:37","date_gmt":"2015-01-27T23:51:37","guid":{"rendered":"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/?p=1808"},"modified":"2015-06-19T09:49:49","modified_gmt":"2015-06-19T09:49:49","slug":"bagging-and-tagging-2","status":"publish","type":"post","link":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/2015\/01\/27\/bagging-and-tagging-2\/","title":{"rendered":"Bagging and Tagging"},"content":{"rendered":"<p>The field work is great but you must always remember that you almost need an equal time to bag and tag your samples than it takes to collect them.  Luis had made a great start but the next morning there was still another 7 sites to process. Now this is when you find out what biologists do in hotel room bathrooms &#8211; yes we turn them into field laboratories.  All tubes have to be pre-labled both externally and by internally by including pencil written tracing paper tags. Then the worms have to be sorted, washed, fixed in medical ethanol and placed in tubes. Here&#8217;s what it looks like:<\/p>\n<p>Bagging and tagging:<\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/labelledtudes\/\" rel=\"attachment wp-att-1812\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/LabelledTudes.jpg?resize=300%2C224\" alt=\"LabelledTudes\" width=\"300\" height=\"224\" class=\"aligncenter size-medium wp-image-1812\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/LabelledTudes.jpg?resize=300%2C224 300w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/LabelledTudes.jpg?w=640 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>Earthworm production line:<\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/earthworm-processing\/\" rel=\"attachment wp-att-1811\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Processing.jpg?resize=300%2C224\" alt=\"Earthworm Processing\" width=\"300\" height=\"224\" class=\"aligncenter size-medium wp-image-1811\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Processing.jpg?resize=300%2C224 300w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Processing.jpg?w=640 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>The earthworm haul:<\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/earthworm-haul\/\" rel=\"attachment wp-att-1810\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Haul.jpg?resize=300%2C224\" alt=\"Earthworm Haul\" width=\"300\" height=\"224\" class=\"aligncenter size-medium wp-image-1810\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Haul.jpg?resize=300%2C224 300w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Earthworm-Haul.jpg?w=640 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>Bagged and ready to go:<\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/bagged-and-ready-to-go\/\" rel=\"attachment wp-att-1809\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Bagged-and-ready-to-go.jpg?resize=300%2C224\" alt=\"Bagged and ready to go\" width=\"300\" height=\"224\" class=\"aligncenter size-medium wp-image-1809\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Bagged-and-ready-to-go.jpg?resize=300%2C224 300w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Bagged-and-ready-to-go.jpg?w=640 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>You may ask and what did we find?&#8230;.well apart from <i>Amynthas<\/i> that is.  Luis did the initial identification and said that we had two earthworm types a <i>Lumbricus terrestrius<\/i> and members of the <i>Allolobophora chlorotica<\/i> complex (we call it a complex because it contains more than one species). All credit to Luis we sent pictures to John (Morgan) our resident expert and he confirmed the preliminary identifications &#8211; but we will not really know until closer anatomic and genetic analysis can be carried out &#8211; but this is what they look like:<\/p>\n<p><i>Lumbricus terrestrius<\/i><\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/worm1\/\" rel=\"attachment wp-att-1817\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm1.jpg?resize=225%2C300\" alt=\"Worm1\" width=\"225\" height=\"300\" class=\"aligncenter size-medium wp-image-1817\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm1.jpg?resize=225%2C300 225w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm1.jpg?w=480 480w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n<p><i>Allolobophora chlorotica<\/i><\/p>\n<p><a href=\"http:\/\/biosi.subsite.cf.ac.uk\/biosi\/kille-morgan\/bagging-and-tagging\/worm2\/\" rel=\"attachment wp-att-1818\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm2.jpg?resize=225%2C300\" alt=\"Worm2\" width=\"225\" height=\"300\" class=\"aligncenter size-medium wp-image-1818\" srcset=\"https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm2.jpg?resize=225%2C300 225w, https:\/\/i0.wp.com\/sites.cardiff.ac.uk\/kille-morgan\/files\/2015\/01\/Worm2.jpg?w=480 480w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The field work is great but you must always remember that you almost need an equal time to bag and tag your samples than it takes to collect them. Luis had made a great start but the next morning there was still another 7 sites to process. Now this is when you find out what<\/p>\n","protected":false},"author":3463,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[3,4,1],"tags":[],"class_list":["post-1808","post","type-post","status-publish","format-standard","hentry","category-azores","category-azores2015","category-uncategorized"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p6t6rP-ta","meta_box":[],"_links":{"self":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/posts\/1808","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/users\/3463"}],"replies":[{"embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/comments?post=1808"}],"version-history":[{"count":1,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/posts\/1808\/revisions"}],"predecessor-version":[{"id":2552,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/posts\/1808\/revisions\/2552"}],"wp:attachment":[{"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/media?parent=1808"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/categories?post=1808"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/sites.cardiff.ac.uk\/kille-morgan\/wp-json\/wp\/v2\/tags?post=1808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}